System adaptability solution

ColumnUltisil® XB-C18(
4.6×150mm,3μm)
Detection210nm
Temperature50℃
Flow Rate1.0ml/min
Injection Volume20ul
Note/

Mobile Phase
Mobile phase A: take 900ml ammonium sulfate buffer, mix it with 100ml acetonitrile, shake it well and degass it by ultrasonic.

Mobile phase B: take 500ml ammonium sulfate buffer, mix it with 500ml acetonitrile, shake it well and degass it by ultrasonic

Time(min)Mobile phase A (%)Mobile phase B (%)
08911
458515
605050
618911
758911

If you have any problem or require further information, please contact info@welchmat.com


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