System adaptability solution

Column | Ultisil® XB-C18( 4.6×150mm,3μm) |
Detection | 210nm |
Temperature | 50℃ |
Flow Rate | 1.0ml/min |
Injection Volume | 20ul |
Note | / |
Mobile Phase
Mobile phase A: take 900ml ammonium sulfate buffer, mix it with 100ml acetonitrile, shake it well and degass it by ultrasonic.
Mobile phase B: take 500ml ammonium sulfate buffer, mix it with 500ml acetonitrile, shake it well and degass it by ultrasonic
Time(min) | Mobile phase A (%) | Mobile phase B (%) |
0 | 89 | 11 |
45 | 85 | 15 |
60 | 50 | 50 |
61 | 89 | 11 |
75 | 89 | 11 |
If you have any problem or require further information, please contact info@welchmat.com
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