System adaptability solution
| Column | Ultisil® XB-C18（ 4.6×150mm，3μm) |
| Detection | 210nm |
| Temperature | 50℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Note | / |
Mobile Phase
Mobile phase A: take 900ml ammonium sulfate buffer, mix it with 100ml acetonitrile, shake it well and degass it by ultrasonic.
Mobile phase B: take 500ml ammonium sulfate buffer, mix it with 500ml acetonitrile, shake it well and degass it by ultrasonic
| Time(min） | Mobile phase A (%) | Mobile phase B (%) |
| 0 | 89 | 11 |
| 45 | 85 | 15 |
| 60 | 50 | 50 |
| 61 | 89 | 11 |
| 75 | 89 | 11 |
If you have any problem or require further information, please contact info@welchmat.com
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