Reference solution
| Column | Ultisil®UHPLC LP-C18(2.1×100mm，1.8μm) |
| Detection | 320nm |
| Temperature | 30℃ |
| Flow Rate | 0.3ml/min |
| Injection Volume | 1ul |
| Note | / |
Mobile Phase
Mobile phase A: take 0.5ml phosphoric acid, add 1000ml Wahaha water, mix well, filter by suction, and degass by ultrasonic. Mobile phase B: take chromatographic grade acetonitrile.
| Time(min） | Mobile phase A (%) | Mobile phase B (%) |
| 0 | 85 | 16 |
| 5 | 84 | 16 |
| 5.1 | 82 | 18 |
| 12 | 82 | 65 |
| 12.1 | 40 | 60 |
| 14 | 40 | 60 |
| 14.1 | 84 | 16 |
| 20 | 84 | 16 |
If you have any problem or require further information, please contact info@welchmat
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