Blank Solution
| Column | Ultisil® AQ-C18(4.6×250mm，5μm) |
| Detection | 205nm |
| Temperature | 25℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Note | / |
Mobile Phase
1.0g/l dipotassium hydrogen phosphate: take 1.0g dipotassium hydrogen phosphate and add it to 1000ml water, Adjust the pH to 6.0 with phosphoric acid, shake well, filter by suction and degassing by ultrasonic. Acetonitrile: take chromatographic grade acetonitrile.
| Time(min） | 1.0g/l dipotassium hydrogen phosphate（%） | Acetonitrile (%) |
| 0.01 | 95 | 5 |
| 20 | 80 | 5 |
| 28 | 80 | 20 |
| 28.1 | 95 | 20 |
| 40 | 95 | 5 |
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