Blank Solution

ColumnUltisil® AQ-C18(4.6×250mm,5μm)
Detection205nm
Temperature25℃
Flow Rate1.0ml/min
Injection Volume20ul
Note/

Mobile Phase
1.0g/l dipotassium hydrogen phosphate: take 1.0g dipotassium hydrogen phosphate and add it to 1000ml water, Adjust the pH to 6.0 with phosphoric acid, shake well, filter by suction and degassing by ultrasonic. Acetonitrile: take chromatographic grade acetonitrile.

Time(min)1.0g/l dipotassium
hydrogen phosphate(%)
Acetonitrile (%)
0.01955
20805
288020
28.19520
40955

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