Sample Solution
| Column | Ultisil XB-C18, 5μm, 4.6×250mm |
| Mobile Phase | Methanol:Acetonitrile:0.1% Sodium Heptanesulfonate:Triethylamine=100:190:710:5(pH2.5) |
| Detection | 240nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Note | Sensitive to injection volume and sample dissolving solvents |
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