Sample Solution
Column | Ultisil XB-C18, 5μm, 4.6×250mm |
Mobile Phase | Methanol:Acetonitrile:0.1% Sodium Heptanesulfonate:Triethylamine=100:190:710:5(pH2.5) |
Detection | 240nm |
Temperature | 40℃ |
Flow Rate | 1.0ml/min |
Injection Volume | 20ul |
Note | Sensitive to injection volume and sample dissolving solvents |
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