Water extraction

ColumnUltisil® Polar-RP(4.6×250 mm,5 μm)
Temperature25℃
Flow Rate1.0ml/min
Injection Volume10ul
Note/

Mobile Phase

A: 0.1% formic acid aqueous solution, B: acetonitrile

Time(min)Mobile phase A(%)Mobile phase B (%)
01000
21100
10982
20955
50595
511000
651000

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