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|Mobile Phase||Mobile phase: take 4.4g dipotassium hydrogen phosphate, add 1000mL water, and then add phosphoric acid|
Adjust the pH to 6.5, filter with suction, mix 630 mL of dipotassium hydrogen phosphate solution with 350 mL of methanol and 20 mL of acetonitrile, and degas by ultrasonic.
Acetonitrile/Methanol/Phosphate = 2/35/63
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