Mixed Standard:

ColumnUltisil® Hilic-NH2, 5μm, 4.6×250mm
Ultisil® AQ-C18, 5μm, 4.6×50mm
Mobile Phase0.01mol/L ammonium dihydrogen phosphate (adjust pH=3.0 with phosphate)- acetonitrile =90/10
Detection210 nm
Temperature40 ℃
Flow Rate1 ml/min
Injection Volume10 µl
NotesSensitive to inject volume and solvent

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