Mixed Standard:
Column | Ultisil® Hilic-NH2, 5μm, 4.6×250mm Ultisil® AQ-C18, 5μm, 4.6×50mm |
Mobile Phase | 0.01mol/L ammonium dihydrogen phosphate (adjust pH=3.0 with phosphate)- acetonitrile =90/10 |
Detection | 210 nm |
Temperature | 40 ℃ |
Flow Rate | 1 ml/min |
Injection Volume | 10 µl |
Notes | Sensitive to inject volume and solvent |
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