Reference solution

Column | Xtimate® C18(4.6×250mm,5μm) |
Mobile Phase | Phosphate buffer salt: take 1.74g anhydrous dipotassium hydrogen phosphate, add 1000ml water to dissolve, and Adjust the pH value of phosphoric acid to 7.0 for ultrasonic degassing. Phosphate buffer / acetonitrile = 65 / 35 |
Detection | 289nm |
Temperature | 30℃ |
Flow Rate | 1.0ml/min |
Injection Volume | 20ul |
Note | / |
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