System suitability solution

ColumnXtimate® C18(4.6×250mm,5μm)
Detection289nm
Temperature40℃
Flow Rate1.0ml/min
Injection Volume20ul
Note/

Mobile Phase
Phosphate buffer salt: take 1.74g of dipotassium hydrogen phosphate, add 1000ml Wahaha water to dissolve, and use phosphoric acid Adjust the pH value to 7.0, filter ultrasonic degassing, and get it.

Time(min)Phosphate buffer (%)Acetonitrile (%)
09010
306040
451585
469010
559010

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