System suitability solution
| Column | Xtimate® C18(4.6×250mm，5μm) |
| Detection | 289nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Note | / |
Mobile Phase
Phosphate buffer salt: take 1.74g of dipotassium hydrogen phosphate, add 1000ml Wahaha water to dissolve, and use phosphoric acid Adjust the pH value to 7.0, filter ultrasonic degassing, and get it.
| Time(min） | Phosphate buffer (%) | Acetonitrile (%) |
| 0 | 90 | 10 |
| 30 | 60 | 40 |
| 45 | 15 | 85 |
| 46 | 90 | 10 |
| 55 | 90 | 10 |
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