Reference
| Column | Ultisil® LP-C18, 5μm, 4.6×250mm |
| Mobile Phase | 10% tetramethylammonium hydroxide: water: acetonitrile =20:380:850, adjust pH to 4.7 with phosphoric acid |
| Detection | 220 nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
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