
Column | Topsil® C8 (4.6×250mm,5μm) |
Mobile Phase | 0.01mol/L disodium hydrogen phosphate solution (adjust pH to 7.6 with phosphoric acid)-acetonitrile (75:25) |
Detection | 230nm |
Temperature | 35℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μL |
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