Systematic solution

ColumnUltisil® LP-C8(
4.6×100mm,3μm)
Detection250nm
Temperature40℃
Flow Rate1.0ml/min
Injection Volume10ul
Note/

Mobile Phase

Phosphate buffer (pH 3.4): 2.04 g of dihydrogen phosphate was called, dissolved in 1000 ml of water, adjusted pH to 3.4 with 1.73 g / L phosphoric acid, mixed with mixed filtration. Mobile phase A: phosphate buffer (pH 3.4) / acetonitrile = 80/20; Mobile phase B: phosphate buffer (pH 3.4) / acetonitrile = 20/80

Time(min)Methanol (%)0.5% Phosphoric
Acid(%)
07525
107525
350100
450100
507525
557525

If you have any problem or require further information, please contact info@welchmat.com.


0 Comments

Leave a Reply

Avatar placeholder

Your email address will not be published. Required fields are marked *