Systematic solution
| Column | Ultisil® LP-C8（ 4.6×100mm，3μm) |
| Detection | 250nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
Phosphate buffer (pH 3.4): 2.04 g of dihydrogen phosphate was called, dissolved in 1000 ml of water, adjusted pH to 3.4 with 1.73 g / L phosphoric acid, mixed with mixed filtration. Mobile phase A: phosphate buffer (pH 3.4) / acetonitrile = 80/20; Mobile phase B: phosphate buffer (pH 3.4) / acetonitrile = 20/80
| Time(min） | Methanol (%) | 0.5% Phosphoric Acid(%) |
| 0 | 75 | 25 |
| 10 | 75 | 25 |
| 35 | 0 | 100 |
| 45 | 0 | 100 |
| 50 | 75 | 25 |
| 55 | 75 | 25 |
If you have any problem or require further information, please contact info@welchmat.com.
0 条评论