| Column | Ultisil® XB-C18（4.6×150mm，3μm) |
| Mobile Phase | Ammonium acetate and sodium perchlorate solution: take 4.0g ammonium acetate and 7.0g sodium perchlorate, add 1300mL. Wahaha dissolves it, adjusts the pH to 2.2 with phosphoric acid, shakes well, and ultrasonically degass. Ammonium acetate sodium perchlorate solution/acetonitrile = 85/15 |
| Detection | 294nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
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