
Column | Ultisil®XS-C18(4.6×250mm,5μm) |
Mobile Phase | Methanol/acetonitrile/water=25/20/55 (adjusted pH=3.0 with glacial acetic acid) |
Detection | 254nm |
Temperature | 30℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 10 μL |
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