Test Sample     Detector: 278nm

Gradient Program

Time(min)A%B%
01000
101000
205050
305050
321000
421000
ColumnWelch Xtimate C18 4.6x150mm,5μm
Mobile PhaseA: 0.025mol/L phosphoric acid solution (take 1.66ml phosphoric acid solution diluted with 1000ml water, then adjust the pH to 3.0 ± 0.1 with triethylamine) – acetonitrile = 87: 13; B: acetonitrile
Detector278nm
Temperature30℃
Flow Rate1ml/min
Injection Volume20ul

If you have any problem or require further information, please contact info@welchmat.com.


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