Test Sample Detector: 278nm
Gradient Program
Time(min) | A% | B% |
0 | 100 | 0 |
10 | 100 | 0 |
20 | 50 | 50 |
30 | 50 | 50 |
32 | 100 | 0 |
42 | 100 | 0 |
Column | Welch Xtimate C18 4.6x150mm,5μm |
Mobile Phase | A: 0.025mol/L phosphoric acid solution (take 1.66ml phosphoric acid solution diluted with 1000ml water, then adjust the pH to 3.0 ± 0.1 with triethylamine) – acetonitrile = 87: 13; B: acetonitrile |
Detector | 278nm |
Temperature | 30℃ |
Flow Rate | 1ml/min |
Injection Volume | 20ul |
If you have any problem or require further information, please contact info@welchmat.com.
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