Test Sample Detector: 278nm
Gradient Program
| Time(min) | A% | B% |
| 0 | 100 | 0 |
| 10 | 100 | 0 |
| 20 | 50 | 50 |
| 30 | 50 | 50 |
| 32 | 100 | 0 |
| 42 | 100 | 0 |
| Column | Welch Xtimate C18 4.6x150mm,5μm |
| Mobile Phase | A: 0.025mol/L phosphoric acid solution (take 1.66ml phosphoric acid solution diluted with 1000ml water, then adjust the pH to 3.0 ± 0.1 with triethylamine) – acetonitrile = 87: 13; B: acetonitrile |
| Detector | 278nm |
| Temperature | 30℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
If you have any problem or require further information, please contact info@welchmat.com.
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