Determination of NMN (Nicotinamide Mononucleotide) Content using Ultisil LP-C18

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ColumnWelch Ultisil®LP-C18（4.6×250mm，5μm)
Mobile PhaseTime 0.05 mol/L Potassium Acetonitrile (%）
Dihydrogen Phosphate(pH6.0)(%)
0 98 2
10 95 5
20 80 20
21 98 2
30 98 2
Column
Temperature		30 ℃
Detection
wavelength:		254 nm
Flow Rate0.8 ml/min
Injection
Volume		20 μL
Note/
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