Blank solution
| Column | Ultisil®XB-C18（4.6×250mm，5μm) |
| Detection | 230nm |
| Temperature | 35℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
Mobile phase A: 1.01g/l sodium heptanesulfonate solution (adjust the pH value to 2.6 with phosphoric acid)
| Time(min） | Mobile phase A (%) | Methanol (%) |
| 0 | 85 | 15 |
| 2 | 85 | 15 |
| 35 | 50 | 50 |
| 40 | 50 | 50 |
If you have any problem or require further information, please contact info@welchmat.com.
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