| Column | Xtimate® C8（4.6×150mm，5μm) |
| Mobile Phase | (1.3g/L sodium dodecyl sulfate in 0.1% phosphoric acid water)/acetonitrile=61/39 |
| Detection | 223nm |
| Temperature | 22℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
| Note | The ratio of organic phase and column temperature have great influence on separation |
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