Nelumbinis Plumula is the green leaf and radicle of Nelumbo Nucifera Gaertn. It is taken from the lotus seed of lotus, which is an aquatic plant and prefers relatively stable calm shallow water, lake, marsh, pond, and is its natural habitat. Lotus seed heart has the effect of clearing the heart and calming the mind, communicating the heart and kidney, astringent essence and hemostasis. It is mainly used for heat into the pericardium, delirium of god, heart and kidney issues, insomnia and spermatorrhea, blood heat and blood vomiting.
According to the method of determination of lotus seed main content in Chinese Pharmacopoeia 2020 edition, Welch Ultisil® XB C18 was used for detection, and the results met the detection requirements.
Mobile phase configuration
Mobile phase: Take 1.02g sodium acetate and 2.0g sodium dodecyl sulfonate (Aladdin), add 500mL ultra-pure water, mix well, then adjust pH to 3.0 with glacial acetic acid to make 0.015mol/L sodium acetate solution, filter. Take the above solution 480mL and chromatographic grade acetonitrile 520mL mixed evenly, ultrasonic degassing, obtained.
The chromatographic conditions
Column: Welch Ultisil® XB C18 (4.6×250mm, 5μm).
Mobile phase: 0.015mol/L sodium acetate solution: 1.02g sodium acetate and 2.0g sodium dodecyl sulfonate were respectively added to 500mL ultrapure water, mixed evenly, then glacial acetic acid was used to adjust the pH to 3.0 to make 0.015mol/L sodium acetate solution, filtered, and obtained.
Acetonitrile /0.015mol/L sodium acetate solution =52/48;
Flow rate: 1.0 mL /min;
Injection volume: 10μL;
Spectrograms and data
Using Welch Ultisil® XB-C18 (4.6×250mm, 5μm), the chromatographic conditions can meet the detection requirements.
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