Dicyandiamide reference solution

ColumnXtimate® C18(4.6×150mm,5μm)
Mobile Phase0.05% sodium heptanesulfonate solution: take 0.5g sodium
heptanesulfonate and add 1000ml water
Adjust the pH to 4.0 with 10% phosphoric acid solution, shake well
and degass with ultrasonic.
Acetonitrile: take chromatographic grade acetonitrile.
0.05% sodium heptanesulfonate solution / acetonitrile = 84 / 16
Detection218nm (related substances) / 233nm (content determination)
Flow Rate1.0ml/min
Injection Volume20ul

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