| Column | Xtimate®NH2（4.6×300mm，5μm)（Article number：00103-21044） |
| Mobile Phase | Measure 750ml of chromatographic grade acetonitrile, add 250ml of water, mix well, and ultrasonically degas. Acetonitrile/Water = 75/25 |
| Detection | RID detector |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 50 μL |
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