Determination of Luteolin in Lonicerae Japonica(Honeysuckle)

Honeysuckle has been known as a good medicine for clearing away heat and detoxification since ancient times. The name “honeysuckle” comes from the compendium of Materia Medica. Because honeysuckle turns white at first and then yellow, it is named honeysuckle. Honeysuckle is a dried flower bud or flower with initial opening of honeysuckle and its congeners. It is sweet, cold and fragrant. It is sweet, cold and heat clearing without hurting the stomach. It is fragrant and can dispel evil. It is used to clear the body heat and silvery rash. It can also relieve the symptoms of fever and sore, such as fever, sore throat and so on.

In this paper, the content of luteolin in honeysuckle was determined by phenyl column according to the conditions of Chinese Pharmacopoeia.

Chromatographic Conditions

Column: Ultisil® Phenyl Ether (4.6×250mm, 5μm);

Mobile phase: AB two-phase gradient elution;

Preparation of mobile phase:

Mobile phase A: 0.5% glacial acetic acid solution, 500ml purified water, adding 2.5ml glacial acetic acid, ultrasonic mixing, extraction filtration, obtained;

Mobile phase B: chromatographic grade acetonitrile, extraction and filtration, available;

Wavelength: 350nm;

Temperature: 30℃.

Spectrograms and data

Reference solution diagram

Sample solution diagram

Results

Welch Ultisil® Phenyl-Ether（4.6 × 250mm，5 μ m) , under this chromatographic condition, it can meet the requirements of detection.

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