| Column | Ultimate® LP-C18（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | 238nm |
| Temperature | 30℃ |
| Flow Rate | 1.5 ml/min |
| Injection Volume | 10 μL |
Mobile Phase:
| Time (min) | 0.1% Phosphoric acid (%) | Acetonitrile (%) |
| 0 | 40 | 60 |
| 7 | 40 | 60 |
| 9 | 35 | 65 |
| 15 | 10 | 90 |
| 20 | 10 | 90 |
| 25 | 40 | 60 |
If you have any problem or require further information, please contact info@welchmat.com.
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