Blank solvent (flow rate: 1.5ml/min)
| Column | Ultisil® LP-C18（ 4.6×250mm，5μm) |
| Detection | 238nm |
| Temperature | 30℃ |
| Flow Rate | 1.5ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
| Time(min） | 0.1% phosphoric acid (%) | Acetonitrile (%) |
| 0 | 40 | 60 |
| 7 | 40 | 60 |
| 9 | 35 | 65 |
| 15 | 10 | 90 |
| 20 | 10 | 90 |
| 25 | 40 | 60 |
If you have any problem or require further information, please contact info@welchmat.com
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