| Column | Xtimate® C18（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | 220nm |
| Temperature | 35℃ |
| Flow Rate | 1.3 ml/min |
| Injection Volume | 10 μL |
Mobile Phase:
| Time (min) | 0.1% Phosphoric acid (%) | Acetonitrile (%) |
| 0 | 75 | 25 |
| 5 | 75 | 25 |
| 30 | 10 | 90 |
| 40 | 10 | 90 |
| 41 | 75 | 25 |
| 50 | 75 | 25 |
If you have any problem or require further information, please contact info@welchmat.com.
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