Reference solution

ColumnBlossmate® ST-C18(
4.6×250mm,5μm)
Mobile PhaseMobile phase A: take 19.06g sodium tetraborate decahydrate, add
1000ml Wahaha water to dissolve, then adjust the pH to 6.1 with
85% phosphoric acid, shake well and degass with ultrasonic.

Mobile phase B: take chromatographic grade methanol and
degassing by ultrasonic.


Mobile phase A / mobile phase B = 1 / 1
Detection214nm
Temperature40℃
Flow Rate1.0ml/min
Injection Volume10ul
NoteThe column temperature will affect the peak shape, reduce the column temperature and increase the peak front delay

If you have any problem or require further information, please contact info@welchmat.com.


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