Reference solution
| Column | Blossmate® ST-C18（ 4.6×250mm，5μm) |
| Mobile Phase | Mobile phase A: take 19.06g sodium tetraborate decahydrate, add 1000ml Wahaha water to dissolve, then adjust the pH to 6.1 with 85% phosphoric acid, shake well and degass with ultrasonic. Mobile phase B: take chromatographic grade methanol and degassing by ultrasonic. Mobile phase A / mobile phase B = 1 / 1 |
| Detection | 214nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | The column temperature will affect the peak shape, reduce the column temperature and increase the peak front delay |
If you have any problem or require further information, please contact info@welchmat.com.
0 条评论