Reference solution

ColumnBlossmate® ST-C18(
Mobile PhaseMobile phase A: take 19.06g sodium tetraborate decahydrate, add
1000ml Wahaha water to dissolve, then adjust the pH to 6.1 with
85% phosphoric acid, shake well and degass with ultrasonic.

Mobile phase B: take chromatographic grade methanol and
degassing by ultrasonic.

Mobile phase A / mobile phase B = 1 / 1
Flow Rate1.0ml/min
Injection Volume10ul
NoteThe column temperature will affect the peak shape, reduce the column temperature and increase the peak front delay

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