| Column | Ultisil® Plus 18 (4.6×150mm，3.5μm) |
| Mobile Phase | pH=6.1 buffer solution (take 34g phosphoric acid, dissolve it in 900ml water, adjust pH=6.1 with concentrated ammonia test solution, add water to 1000ml)/methanol/acetonitrile=77/8/15 |
| Detection | 210nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
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