Gradient Program

ColumnXtimate® C18, 4.6×250 mm, 5 μm
Mobile PhaseA: ammonium perchlorate acetate solution (take 4.0g of ammonium acetate and 7.0g of sodium perchlorate, add 1300 mL of water to dissolve, and use Phosphoric acid adjusted pH to 2.2) – acetonitrile =85:15; B: acetonitrile
Detection294 nm
Temperature40℃
Flow Rate1.0 mL/min
Injection Volume10 µL

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