|Column||Xtimate® C18, 4.6×250 mm, 5 μm|
|Mobile Phase||A: ammonium perchlorate acetate solution (take 4.0g of ammonium acetate and 7.0g of sodium perchlorate, add 1300 mL of water to dissolve, and use Phosphoric acid adjusted pH to 2.2) – acetonitrile =85:15; B: acetonitrile|
|Flow Rate||1.0 mL/min|
|Injection Volume||10 µL|
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