Test Solution

ColumnUltisil®ALK-C18(4.6×250mm,5um)
Mobile PhaseAmmonium acetate buffer: take 4.0g ammonium acetate and 7.0g
sodium perchlorate monohydrate, add 1300mL
In water, adjust the pH to 2.2 with phosphoric acid, shake well, and
ultrasonically degas.
Ammonium acetate buffer/acetonitrile = 83/17
Detection332nm
Temperature40℃
Flow rate1.0ml/min
Injection Volume5ul
Note\

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