
Column | Ultisil® Plus C18(4.6×250mm,4.0μm) |
Mobile Phase | Sodium heptanesulfonate buffer salt: take 6.8g potassium dihydrogen phosphate and 0.85g sodium heptanesulfonate, add 1000mL Wahaha water, shake well, adjust the pH to 2.8 with phosphoric acid, and degas by ultrasonic. Acetonitrile: Take chromatographic grade acetonitrile and get it. Sodium heptane sulfonate buffer/acetonitrile = 93.5/6.5 |
Detection | 200nm |
Temperature | 30℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 10 μL |
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