ColumnUltisil® Plus C18(4.6×250mm,4.0μm)
Mobile PhaseSodium heptanesulfonate buffer salt: take 6.8g potassium dihydrogen phosphate
and 0.85g sodium heptanesulfonate, add
1000mL Wahaha water, shake well, adjust the pH to 2.8 with phosphoric acid, and
degas by ultrasonic.
Acetonitrile: Take chromatographic grade acetonitrile and get it.
Sodium heptane sulfonate buffer/acetonitrile = 93.5/6.5
Flow Rate1.0 ml/min
Injection Volume10 μL

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