Resolution solution

ColumnUltisil®Plus C18(
4.6×250mm,4.0μm)
Mobile PhaseSodium heptanesulfonate buffer salt: take 6.8g potassium dihydrogen
phosphate and 0.85g sodium heptanesulfonate, add them into 1000ml water, shake them well, adjust the pH to 2.8 with phosphoric acid,
and degass them by ultrasonic.
Acetonitrile: take chromatographic grade acetonitrile.

Sodium heptanesulfonate buffer / acetonitrile = 93.5 / 6.5
Detection200nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume10ul
Note/

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