Sample
| Column | Welch Ultisil XB-C8 4.6×250mm, 5μm |
| Mobile Phase | A: 20g ammonium sulfate mixed with 900mL water and 1000mL acetonitrile, adjust the pH to 2.3 with sulfuric acid; B: acetonitrile: Water = 80:20; |
| Detector | 214nm |
| Temperature | 25℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
Mobiel Phase
| Time(min) | A% | B% |
| 0 | 100 | 0 |
| 9 | 70 | 30 |
| 40 | 40 | 60 |
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