Sample

ColumnWelch Ultisil XB-C8 4.6×250mm, 5μm
  Mobile PhaseA: 20g ammonium sulfate mixed with 900mL water and 1000mL acetonitrile, adjust the pH to 2.3 with sulfuric acid; B: acetonitrile: Water = 80:20;
Detector214nm
Temperature25℃
Flow Rate1ml/min
Injection Volume20ul

Mobiel Phase

Time(min)A%B%
01000
97030
404060

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