| Column | Ultisil®SiO2（4.6×250mm，5μm，300Å） |
| Mobile Phase | See the table below for details |
| ELSD | Carrier gas pressure: 3.5 Bar Temperature: 100℃ |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
Mobile Phase:
Phase A: n-hexane (with 0.04% triethylamine)/isopropanol/13% acetic acid solution=40/57/3 Phase B: n-hexane (with 0.04% triethylamine)/isopropanol/13% acetic acid solution=40/50/10
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 100 | 0 |
| 10 | 0 | 100 |
| 18 | 0 | 100 |
| 18.1 | 100 | 0 |
| 30 | 100 | 0 |
If you have any problem or require further information, please contact info@welchmat.com.
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