ColumnUltisil®SiO2(4.6×250mm,5μm,300Å)
Mobile PhaseSee the table below for details
ELSDCarrier gas pressure: 3.5 Bar Temperature: 100℃
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

Mobile Phase:

Phase A: n-hexane (with 0.04% triethylamine)/isopropanol/13% acetic acid solution=40/57/3 Phase B: n-hexane (with 0.04% triethylamine)/isopropanol/13% acetic acid solution=40/50/10

Time (min)Mobile phase A(%)Mobile phase B(%)
01000
100100
180100
18.11000
301000

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