System Solution Diagram
| Column | Ultisil® AQ C18(4.6×250mm，5μm) |
| Detection | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
| Time(min） | Mobile phase A (%) | Mobile phase B (%) |
| 0 | 99 | 1 |
| 30 | 50 | 50 |
| 40 | 0 | 100 |
| 50 | 0 | 100 |
| 51-65 | 99 | 1 |
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