Full scale diagram

ColumnUltisil®LP-C18(4.6×300mm,5μm)
Mobile Phase0.025mol/L Ammonium acetate solution (weigh 1.925g ammonium
acetate, add 900mL water to dissolve, adjust pH to 3.95 with glacial acetic acid, dilute to 1000mL with water) – methanol (95:5)
Detection277nm
Temperature35℃
Flow Rate1.0ml/min
Injection Volume10ul
Note/

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