| Column | Ultisil® LP-C18（4.6×300mm，5μm) |
| Mobile Phase | 0.025mol/L ammonium acetate solution (weigh 1.925g of ammonium acetate, add 900mL of water to dissolve, adjust pH to 3.95 with glacial acetic acid, dilute to 1000mL with water)-methanol (95:5) |
| Detection | 277nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
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