| Column | Ultisil® HILIC-NH2（4.6×250mm，5μm) |
| Mobile Phase | 0.01mol/L sodium dihydrogen phosphate/acetonitrile=25/75 |
| Detection | Differential detector temperature: 35℃ |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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