ColumnUltisil® Hilic-NH2(4.6×250mm,5μm)
Mobile PhasePhosphate buffer solution: take 6.81g of potassium dihydrogen phosphate, add
1000mL of Wahaha water, mix well and filter, adjust the pH to 4.7 with sodium
hydroxide solution, and degas by ultrasonic.
Phosphate buffer (pH=4.7)/acetonitrile=35/65
Detection205nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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