| Column | Ultisil®PLUS C18 (4.6×250 mm，5 μm) |
| Mobile Phase | See the table below for details |
| Detection | 297nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
Mobile Phase:
Mobile phase A: water-triethylamine (500:0.175) Mobile phase B: methanol-triethylamine (500:0.175)
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 40 | 60 |
| 12 | 40 | 60 |
| 20 | 10 | 90 |
| 25 | 10 | 90 |
| 26 | 40 | 60 |
| 31 | 40 | 60 |
If you have any problem or require further information, please contact info@welchmat.com.
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