Related Substances Reference

ColumnTopsil C18, 4.6×150mm, 5μm
Mobile PhasePhosphate buffer(68g KH2PO4 dissolved in 1000ml water, adjust pH to 3.5 with phosphoric acid) : Acetonitrile : Water = 2:43:55
Detection233nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume20ul
NoteSensitive to injection volume

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