Test sample mixed with standard solution
| Column | Welch Ultisil LP-C18 4.6×250mm，5μm |
| Mobile Phase | Mobile phase A: acetonitrile, Mobile phase B: 0.5% tetrabutylammonium hydroxide solution (10% concentration);adjust pH to 2.0 with phosphoric acid |
| Detector | 225 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20µl |
| Notes | Sensitive to injection volume and dissolved sample solvent |
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