Test sample mixed with standard solution

ColumnWelch Ultisil LP-C18 4.6×250mm,5μm
Mobile PhaseMobile phase A: acetonitrile, Mobile phase B: 0.5% tetrabutylammonium hydroxide solution (10% concentration);adjust pH to 2.0 with phosphoric acid
Detector225 nm
Flow Rate1.0 ml/min
Injection Volume20µl
NotesSensitive to injection volume and dissolved sample solvent

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