Sample solution

ColumnUltisil XB-C18 4.6×250 mm, 5 μm
Mobile Phase(0.01mol/L potassium dihydrogen phosphate buffer solution, adjust pH to 7.0 by 0.1mol/L sodium hydroxide solution): Methanol=40:60
Detection254 nm
Temperature30°C
Flow Rate1.0 ml/min
Injection Volume20 µl

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