| Column | Topsil ® C8（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | 216nm |
| Temperature | 50℃ |
| Flow Rate | 1.2 ml/min |
| Injection Volume | 20 μL |
Mobile Phase:
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 90 | 10 |
| 25 | 40 | 60 |
| 30 | 10 | 90 |
| 35 | 10 | 90 |
| 37 | 90 | 10 |
| 45 | 90 | 10 |
If you have any problem or require further information, please contact info@welchmat.com.
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