
Column | Topsil ® C8(4.6×250mm,5μm) |
Mobile Phase | See the table below for details |
Detection | 216nm |
Temperature | 50℃ |
Flow Rate | 1.2 ml/min |
Injection Volume | 20 μL |
Mobile Phase:
Time (min) | Mobile phase A(%) | Mobile phase B(%) |
0 | 90 | 10 |
25 | 40 | 60 |
30 | 10 | 90 |
35 | 10 | 90 |
37 | 90 | 10 |
45 | 90 | 10 |
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