Test Sample
Column | Xtimate® C18, 4.6×100 mm, 3μm |
Detection | 215nm |
Temperature | 35℃ |
Flow Rate | 1.0 mL/min |
Injection Volume | 8 µL |
Mobile Phase
Time(min) | Dipotassium hydrogen phosphate (pH8.9) | Acetonitrile |
0 | 55 | 45 |
4 | 55 | 45 |
7 | 30 | 70 |
14 | 30 | 70 |
18 | 55 | 45 |
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