| Column | Ultisil® C18（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | 230nm |
| Temperature | 25℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
Mobile Phase：
| Time (min) | 0.1% phosphoric acid (%) | Methanol (%) |
| 0 | 65 | 35 |
| 45 | 59 | 41 |
| 50 | 35 | 65 |
| 60 | 35 | 65 |
| 61 | 65 | 35 |
| 75 | 65 | 35 |
If you have any problem or require further information, please contact info@welchmat.com.
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