Sample Solution
| Column | Xtimate C18, 5μm, 4.6×250mm |
| Detection | 228 nm |
| Temperature | 45℃ |
| Flow Rate | 1.5 ml/min |
| Injection Volume | 10 µl |
Mobile Phase
A: 2.72g /L potassium dihydrogen phosphate solution 1000ml add 1ml triethylamine, adjust pH= 3.0 with phosphoric acid B: acetonitrile methanol =4:1
| Time(min) | A (%) | B (%) |
| 0 | 70 | 30 |
| 25 | 70 | 30 |
| 35 | 40 | 60 |
| 40 | 30 | 70 |
| 42 | 70 | 30 |
| 50 | 70 | 30 |
If you have any problem or require further information, please contact info@welchmat.com.
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