Reference solution
| Column | Xtimate® C18(4.6×250mm，5um) |
| Detection | 270nm |
| Temperature | 20℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | \ |
Mobile Phase
| Time(min) | Acetonitrile | Water |
| 0 | 24 | 76 |
| 30 | 26 | 74 |
| 31 | 45 | 55 |
| 45 | 47 | 53 |
| 45.1 | 24 | 76 |
| 60 | 24 | 76 |
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