Determination of Icariin in Epimedium by Xtimate C18

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Reference solution

ColumnXtimate® C18(4.6×250mm,5um)
Detection270nm
Temperature20℃
Flow Rate1.0ml/min
Injection Volume10ul
Note\

Mobile Phase

Time(min)AcetonitrileWater
02476
302674
314555
454753
45.12476
602476

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