Reference solution

ColumnBoltimate EXT-C18 (150 x 4.6)mm 2.7µ
RTabout 3.00 min
Temperature25°C
Flow Rate1.0ml/min
Injection Volume25 to 75 µL
Wave length248 nm
Run Time35 min for standard and resolution solution

Results

% RSD1.24% 
Tailing factor1.39
Theoretical Plates8133 
Maximum Back Pressure2843 psi
Resolution between prednisolone and hydrocortisone1.18
Resolution between Hydrocortisone and Cortisone0.98

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