Blank solution
| Column | Ultisil®XB-C18（4.6×250mm，5μm) |
| Detection | 210nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 5ul |
| Note | / |
Mobile Phase
Mobile phase A: take 1g sodium bicarbonate, add 1000ml water, mix well, filter, ultrasonic degassing.
Mobile phase B: take chromatographic grade acetonitrile.
| Time(min) | Mobile Phase A(%) | Mobile Phase B(%) |
| 0 | 95 | 5 |
| 4 | 70 | 30 |
| 20 | 70 | 30 |
If you have any problem or require further information, please contact info@welchmat.com
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