Glyphosate is an herbicide. It is applied to the leaves of plants to kill both broadleaf plants and grasses. The sodium salt form of glyphosate is used to regulate plant growth and ripen specific crops.

Glyphosate was first registered for use in the U.S. in 1974. Glyphosate is one of the most widely used herbicides in the United States. People apply it in agriculture and forestry, on lawns and gardens, and for weeds in industrial areas. Some products containing glyphosate control aquatic plants.

Chromatographic Conditions

 ● Column: Blossmate® SAX (4.6×250 mm,5μm).

 ● Mobile phase: buffer-methanol =97-3(adjust pH to 1.90)

 ● Wavelength: 195nm

 ● Column temp: 25℃

 ● Flow rate: 1.5 ml/min

 ● Injection: 20 ul

 ● Note: adjust pH after mixing mobile phase

 Chromatogram and Data

 ● Reference solution:

 ● Overlapped chromatogram:

 Conclusion:

Using Ultisil® PFP (3um, 4.6×150mm) under this chromatographic condition, can meet the test requirements.

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